Abstract : The macro invertebrate fauna and physico-chemical parameters in the down stream of Dikhow River was studied between January and November, 2011. A total of 13 species of benthic invertebrate fauna belonging to three phyla (Annelida, Arthropoda and Mollusca), five classes (Hirudinea, Gastropoda, Bivalvia, Crustacea, Insecta) and thirteen families (Hirudinidae, Physidae, Anomidae, Gammaridae,Panaediae, Baetidae Aeshnidae, Belostometidae, Hydrophilidae, Chaoboridae, Chironomidae) were found in the Dikhow River during the study. Gastropoda was predominant (25%) followed by Crustacea, Bivalvia and Hirudinidae with percentage composition of 17%, 14.4% and 9.5% respectively. Among Insecta, Dipteran midges (Chaoboridae) with 8.6% was the dominant group. Macroinvertebrates tolerance values ranged between 4 and10 and family biotic index values between 6.04 and 7.31